Virtual Lab: Liquid-Liquid Extraction

Separation and Identification of a Binary Mixture: Ionizable Acid and Non-ionizable Compound

Liquid-liquid extraction is a very common technique used in multiple fields from quality analysis of pharmaceutical products to refining chemicals on an industrial scale. This method uses the varying acid-base and solubility properties of organic molecules to separate them. In this virtual lab, you will perform the separation of an unknown solid mixture composed of an ionizable acid and a non-ionizable (neutral) compound. The lab will randomly assign you an unknown mixture to work with.

As you work through this simulation, you will:

• Make choices, such as which reagent is appropriate or which isolation method to use. In doing so, you will be applying experimental design and identifying gaps in your knowledge in this safe space.
• View actual experimental techniques via videos taken in the laboratory of all the major steps of this procedure.
• Finally, you will make a conclusion about the identity of both components in your mixture based on each component’s melting point.

Before You Begin

Review the Liquid-Liquid Extraction Laboratory Unit so you feel confident with concepts of acid-base chemistry, laboratory equipment, and techniques. This virtual lab is for you to make intentional decisions and not ‘guess’ what the right answer would be! If you struggle with decisions when completing the lab, review relevant sections of this laboratory unit again or reach out for help.

Complete the associated Pre-Laboratory Quiz. This quiz will test your understanding of the above laboratory unit.

Set aside 60 minutes to complete the virtual laboratory.